BamHI, FastDigest™, fermentas (2023)


These terms and conditions cover all sales of products and services by VWR International Ltd (VWR) in the Republic of Ireland and Northern Ireland and any information and advice given whether charged for or not. No variation in these terms and conditions will apply unless agreed by VWR in writing. Orders are accepted only on the condition that no conditions of purchase shall apply.


VWR has the right to change the prices shown in catalogues and price lists at any time and to invoice at prices current at the date of receipt of an order.

‘Price on application’ (POA) quotations and all other quotations will be valid for 30 days from the date of the quotation, unless otherwise notified by VWR.

All prices in catalogues and quotations are net of Value Added Tax and any other taxes or duties. These will be charged separately, if applicable.

VWR reserve the right to apply a freight charge on all deliveries. Our stated prices are for goods which are delivered on the ground floor, without unpacking and installation. Any additional freight and handling service related to installation is subject to an additional charge.

A minimum order charge of €30/£30 may apply to orders less than €100/£100.

General public prices are available on VWR’s website. In order to consult specific customer applicable prices, each customer is invited to log in via their personalized profile or to contact VWR to obtain a specific price offer.

Terms of Payment

Unless otherwise agreed in writing payment in full is due within 30 days from date of invoice. VWR has the right to charge interest on all overdue sums at the rate of five percent per annum over the base lending rate of the Bank of Ireland calculated at the date of actual payment.

Customers who exceed their credit limits, or who have an unsatisfactory situation regarding their credit standing, will be asked to pay in advance for additional products until the account is settled.

VWR reserves the right to request an instalment before execution of an order if deemed necessary.

Delivered products will remain the property of VWR until paid for.

VWR has the right to deliver an order in instalments, and the customer is required to pay the invoice for each instalment within 30 days from date of invoice.

In the event of late payment, VWR may suspend any outstanding order, without prejudice to any other course of action, and may claim penalties for late payment at the ECB rate applicable at the 1st January or at the 1st July + 10 points due the day following the payment date plus a fix indemnity for recovery costs.

Products & Use

The products we supply are intended for use in accordance with the documentation and manufacturer specifications under the user’s sole responsibility.

The specifications and figures contained in our catalogues are provided as indications only, and are not of a contractual character. VWR reserves the right to change its articles without advance notice according to improvements which might be required by progress in the applicable technologies.

VWR does not control and inform changes to product specifications. For certain products and under specific conditions, a change control agreement could be signed between the parties.
Products supplied by VWR cannot be used as active pharmaceutical ingredients (APIs) in human or veterinary medicine because, as a distributor, VWR does not fulfil the requirements laid down in the guidelines for GMP medicinal products for human and veterinary use.

Customers using products from VWR in a pharmaceutical, cosmetic, food, pesticide or any other regulated application must carry out their own internal checks and controls to ensure that the items are of an appropriate quality/ grade and in compliance with their local laws and regulations (e.g. European Pharmacopoeia) etc. Please contact VWR if you have any questions about your intended use and if the product you wish to purchase is suitable for that purpose. In no event shall VWR be liable if a customer uses a product that does not fit with the intended application.

It is customer's responsibility to comply with all applicable health, safety and other regulations and to take appropriate steps in relation to the storage, handling, sale and use of the products.


Orders are definitive only if confirmed in writing by VWR, if customer cancels an order, VWR may invoice a cancellation fee. Certain orders (non-catalogue products) are not cancellable.

VWR recommends using the numbers and designations of our catalogues or of our offer. In the event of imprecise designation, if VWR must ourselves make a choice, VWR do not bear any responsibility therefore, and the costs of return for non-conformity shall be covered by the purchaser.

Delivery – Shipment of Products

VWR selects the means of shipment that it finds appropriate for its customer.

The products listed in the ADR (international regulation on the carriage of hazardous merchandise) and marked in VWR catalogues with distinctive signs are shipped only under certain conditions.

For products that requires special carriage or special packing, and for heavy and bulky goods, as well as for express delivery, the customer will be billed additional charges. It is the Customer's responsibility to ensure that spaces are dimensioned (staircase, door, room, etc.) in order to receive the ordered material.

The sale of alcohol is subject to very strict regulation with which the customer must comply. The office of the Revenue Commissioners inspects the sale of alcohol. To that end, prior to any delivery, the purchaser must provide VWR with a copy of the alcohol purchase authorization remitted by the Revenue Commissioner.

The date for delivery specified in VWR documents (quotation, contract, acknowledgement receipt of order etc) are only indicative. No penalty for late delivery, or damages, shall be claimed in the event that the delivery dates are exceeded. Delivery occurs only if the customer is currently in good standing regarding its obligations vis-a-vis VWR.

Claims & Return of Damaged or Unsuitable Goods

Any claims for shortages must be made within 7 working days of a delivery.

Any claims for non-ordered items or non-delivery must be made within 7 working days of receiving the invoice.

No goods may be returned to VWR without the authorisation of VWR.

Authorisation to return products damaged during delivery must be requested within 3 working days of delivery. VWR has the right to repair and return damaged products.

Authorisation for the return of products which fail to meet current published manufacturers specifications must be requested in writing within 28 days of delivery. VWR will assist customers, at customers’ expense, to obtain any manufacturer’s warranty consistent with that granted to VWR.

Authorisation for the return of products, other than those damaged during delivery, delivered in error or those that do not meet specification, must be requested within 28 days of delivery. Credit (less a handling charge of 20% of the invoice value of all products returned subject to a minimum charge of €30/£30) will be given for those products authorised for return which are unused and in re-saleable condition (including original packaging) other than those in the categories shown:

  • open chemicals or diagnostics
  • refrigerated or other perishables
  • items with an expired shelf life or an expiration date too short for resale
  • any article that has been delivered direct by a third-party supplier
  • discontinued items
  • items not purchased from VWR.

Authorisation will be subject to the condition that the products are returned, with the necessary paperwork (detailing returns number, decontamination certificate, if applicable) to VWR’s distribution centre in Dublin by prior arrangement.
Non-core VWR catalogue products and custom-made products that are ordered on the behalf of the customer, and/or have been delivered on our behalf by a third-party supplier will not be accepted back at the VWR’s distribution centre.


New materials and equipment sold by VWR are covered, unless otherwise stated, against any manufacturing defects for a period of 12 to 24 months from:

- technical acceptance of installed equipment;
- delivery of non-installed equipment.

VWR’s warranty applies exclusively in the event of deficiencies resulting from a defect in design or from a hidden defect affecting the goods delivered and rendering it unfit for use.

It is strictly limited to repair of the apparatus in VWR’s workshops or to the customer. If the repair is not possible, it is limited to the replacement of non-compliant or defective good. By express agreement, absolutely excluded from VWR warranty are any damages, particularly for labor costs, loss of earnings, disturbance of business, etc. The spare parts are under warranty 3 months after their installation if such installation was performed by a person certified by VWR.

For chemical products, the provided warranty extends only on the condition that the packages are stored in proper conditions of conservation.

VWR hereby disclaims all other warranties or guarantees with respect to the subject matter, whether statutory, written, oral, express or implied including without limitation, any warranty of merchantability, suitability or fitness for a particular purpose.
In the event of a dispute, an action for indemnity should be instituted within one year after delivery.

Maintenance After Warranty

After expiration of the warranty period or at point of sale, VWR may offer a maintenance service, in which case VWR will propose to the client a maintenance contract adapted to its needs.

Title and Risk

The risk in the products supplied by VWR shall pass to the customer on completion of delivery.

Title to the products shall not pass to the customer until VWR receives payment in full and any other goods that VWR has supplied to the customer in respect of which payment has become due, in which case title to the products shall pass at the time of payment of all such sums.

Until title to the products has passed to the customer, the customer shall:

  • store the products separately from all other products held by the customer so that they remain readily identifiable as VWR's property;
  • not remove, deface or obscure any identifying mark or packaging on or relating to the products;
  • maintain the products in satisfactory condition and keep them insured against all risks for their full price on VWR's behalf from the date of delivery;
  • give VWR such information relating to the products as VWR may require from time to time.

Liability of VWR for any loss or damage suffered by a customer and arising by reason of defects in the products or otherwise howsoever is limited to the invoice price of the products in respect of or in relation to which loss or damage is claimed. Any liability accepted by VWR under these terms and conditions is in lieu of any warranty or condition implied by law as to the quality or fitness for any particular purpose of the products and save as provided in these terms and conditions VWR will not be under any liability, whether in contract, tort, breach of statutory duty or otherwise in respect of defects in products delivered or for any injury (other than death or personal injury caused by VWR negligence as defined in the Unfair Contract Terms Act 1977) damage or loss resulting from such defects or from any information, service or advice provided by VWR or its employees, contractors or agents. In no event shall VWR be liable for any consequential loss whether or not resulting from force majeure. The customer indemnifies VWR against any claims from its employees, contractors or agents.

The customer is required to ensure that that the use of any products supplied by VWR do not infringe third party intellectual property rights.

Force Majeure

Fires, floods, machine breakage, riots, war, shortage of supplies and any other incidents which prevent or reduce the manufactures and services are deemed to be events of force majeure and release VWR from the obligation of delivery.

Intellectual Property Rights

All intellectual property rights in or arising out of or in connection with the products and services shall be owned by VWR.

The customer acknowledges that, in respect of any third party Intellectual Property Rights, the Customer's use of any such Intellectual Property Rights is conditional on VWR obtaining a written licence from the relevant licensor on such terms as will entitle VWR to license such rights to the Customer.

All supplier materials are the exclusive property of VWR.


A customer shall keep in strict confidence all technical or commercial know-how, specifications, inventions, processes or initiatives which are of a confidential nature and have been disclosed to by VWR, its employees, agents or subcontractors, and any other confidential information concerning VWR’s business, its products and services which the customer may obtain. The customer shall only disclose such confidential information to those of its employees, agents and subcontractors who need to know it for the purpose of discharging the customers obligations under these terms & conditions, and shall ensure that such employees, agents and subcontractors comply with the obligations set out in this clause as though they were a party to these terms & conditions.

Data Protection

VWR International protects the Customer's personal data. The Customer has the right to access, modify, rectify and delete the personal data that he / she communicates to VWR. The Customer may exercise this right by sending a written request to VWR.

Waste Management/Treatment

In order to fight against pollution and ensure to comply with applicable laws, VWR recycles its waste furniture equipment, electrical and electronic equipment by contributing financially to eco-organizations.


For export sales, our prices are specified.

These General Terms and Conditions of Sale apply to sales in Ireland. If the Customer wishes to export the products, he undertakes to approach the export department of VWR. Any export of VWR product must be done in compliance with the applicable regulations (including export control and compliance with anti-corruption laws) and under its own responsibility. The products we commercialize are packaged for shipments to Ireland. It is the responsibility of the Customer, in the event of export and under his responsibility, to ensure that the repackaging he carries out complies with the regulations.

For export sales, prices are stipulated ex works. A minimum of order is required. Below this amount, a flat rate for processing fees will be billed to the Customer.


Customer represents that it is familiar with and understands the terms of the U.S Foreign Corrupt Practices Act of 1977 (“US FCPA”) and that customer will comply with the US FCPA and all other applicable anti-bribery or anti-corruption laws and regulations of any other country or jurisdiction which are applicable to the parties’ business activities.

Health & Safety

It is customer's responsibility to comply with all applicable health, safety and other regulations and to take appropriate steps in relation to the storage, handling, sale and use of the products.

In view of the hazards of certain chemicals and apparatus the customer must ensure that the products purchased are in a safe condition and that a safe system of work is in place considering all available information. None of the products sold are intended for human consumption unless otherwise clearly stated.

In view of the wide range of uses of chemicals and apparatus, the customer will be solely responsible for determining the suitability and specification of products, services, information and advice for its purposes. VWR has the right, without notice, to supply product of different specifications, sources, and pack size to that published or ordered.

Governing Law

These terms & conditions, and any dispute or claim (including non-contractual disputes or claims) arising out of or in connection with it or its subject matter or formation shall be governed by, and construed in accordance with the law of Ireland.


Each party irrevocably agrees that the courts of Ireland shall have exclusive jurisdiction to settle any dispute or claim (including non-contractual disputes or claims) arising out of or in connection with the contract or its subject matter or formation.


What are Fermentas FastDigest enzymes? ›

FastDigest enzymes in combination with FastDigest (Green) Buffer are designed to eliminate star activity: Short incubation time (5–15 min), no need for prolonged digestions. Universal buffer with optimal pH and salt concentration for all enzymes.

What buffer and temperature is best to use with BamHI? ›

Thermo Scientific BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer.

What is FastDigest restriction digestion? ›

Thermo Scientific FastDigest restriction enzymes are an advanced line of enzymes which offer: Complete digestion in 5–15 minutes. 100% buffer compatibility with downstream applications. Direct loading on gels.

What is the protocol of BamHI? ›

Recommended Protocol for Digestion

Add: nuclease-free water 16 µL 10X Buffer BamHI 2 µL DNA (0.5-1 µg/µL) 1 µL BamHI 0.5-2 µL*,** • Mix gently and spin down for a few seconds. Incubate at 37°C for 1-16 hours**. The digestion reaction may be scaled either up or down.

What is an example of enzymes in digestion? ›

Types of Digestive Enzymes

The main digestive enzymes made in the pancreas include: Amylase (made in the mouth and pancreas; breaks down complex carbohydrates) Lipase (made in the pancreas; breaks down fats) Protease (made in the pancreas; breaks down proteins)

What are fast restriction enzymes? ›

FastDigest® restriction enzymes are an advanced line of restriction enzymes for rapid DNA digestion. All FastDigest® enzymes are 100% active in the universal FastDigest® Green buffer and are able to digest DNA in 5-15 min.

What happens if you add too much restriction enzyme? ›

Incomplete digestion is a frequently encountered issue when using restriction endonucleases. Incomplete digestion may occur when too much or too little enzyme is used. The presence of contaminants in the DNA sample can inhibit the enzymes, also resulting in incomplete digestion.

How do you know which buffer solution is best? ›

A good buffer mixture should have about equal concentrations of both of its components. As a rule of thumb, a buffer solution has generally lost its usefulness when one component of the buffer pair is less than about 10% of the other.

How do you inactivate BamHI? ›

Heat inactivation Up to 10 Units BamH I / µg DNA can be heat-inacti- vated by 15 min incubation at 65°C, higher enzyme concentrations can no more be completely inactivated under these conditions.

How long do you run a restriction digest for? ›

For diagnostic digests, 1-2 hours is often sufficient. For digests with >1 µg of DNA used for cloning, it is recommended that you digest for at least 4 hours.

How long should a restriction digest take? ›

Typically, a restriction digest involves the incubation of 1 µl of enzyme with 1 µg of purified DNA in a final volume of 50 µl for 1 hour. However, to speed up the screening process, choose a Time-Saver™ qualified enzyme for 5-15 minute digestion reactions.

How much enzyme do you add for restriction digest? ›

In general, we recommend 5–10 units of enzyme per µg DNA, and 10–20 units for genomic DNA in a 1 hour digest.

How does BamHI work? ›

BamHI is an extraordinarily unique molecule in that it undergoes a series of unconventional conformational changes upon DNA recognition. This allows the DNA to maintain it's normal B-DNA conformation without distorting to facilitate enzyme binding.

What type of enzyme is BamHI? ›

BamHI is a type II restriction enzyme derived from Bacillus amyloliquefaciens. Like all Type II restriction endonucleases, it is a dimer and the recognition site is palindromic and 6 bases in length. It recognizes the DNA sequence of G'GATCC and leaves an overhang of GATC which is compatible with many other enzymes.

What bacteria is BamHI from? ›

BamHI, from Bacillus amyloliquefaciens H, is a type II restriction-modification system recognizing and cleaving the sequence G--GATCC.

What are the 3 main enzymes found in the stomach? ›

These digestive enzymes are categorized based on the reactions they help catalyze:
  • Amylase breaks down starches and carbohydrates into sugars.
  • Protease breaks down proteins into amino acids.
  • Lipase breaks down lipids, which are fats and oils, into glycerol and fatty acids.
Mar 19, 2022

What are the 4 main digestive enzymes? ›

The 4 main digestive enzymes are carbohydrases, proteases, lipases, and nucleases.

What food is high in enzymes? ›

Foods that contain natural digestive enzymes include pineapples, papayas, mangoes, honey, bananas, avocados, kefir, sauerkraut, kimchi, miso, kiwifruit and ginger. Adding any of these foods to your diet may help promote digestion and better gut health.

Which restriction enzyme is best? ›

The best characterized and most frequently used restriction enzymes are the classical Type II class. These enzymes recognize specific 4 to 8 nucleotide sequences that are typically palindromic and cleave within the recognition site leaving sticky (5′ or 3′ overhangs) or blunt ends.

What is the most used restriction enzyme? ›

One of the most important and most widely used restriction enzymes is EcoRI. EcoRI refers to Escherichia coli's first restriction enzyme, Strain RY13. There are more than 400 known restriction enzymes in microscopic organisms like bacteria that distinguish and cut more than 100 diverse DNA sequences.

What are Type 1 and Type 2 restriction enzymes? ›

Today, scientists recognize three categories of restriction enzymes: type I, which recognize specific DNA sequences but make their cut at seemingly random sites that can be as far as 1,000 base pairs away from the recognition site; type II, which recognize and cut directly within the recognition site; and type III, ...

How do you know if restriction enzymes are working? ›

Check the restriction enzyme's methylation sensitivity. If the restriction enzyme is inhibited by methylation of its recognition site, look for a neoschizomer or isoschizomer that is not affected by methylation (e.g., MvaI for EcoRII). To avoid DNA methylation, propagate plasmids in E. coli hosts that are dam/dcm.

Why didn t my restriction digest work? ›

The restriction enzyme tube or reaction buffer tube may be contaminated with a second enzyme. This can happen where the same reaction buffer is used for multiple different enzymes. Try a fresh tube of enzyme or reaction buffer. Check for contamination by using a fresh DNA preparation.

How do you get rid of restriction enzymes? ›

Restriction enzymes are commonly inactivated by a heat treatment after digestion is complete. However, heat tolerance varies between enzymes, and in some cases is insufficient to completely inactivate particular restriction enzymes.

What pH do most buffers work best at? ›

1. A pKa between 6 and 8. Most biochemical experiments have an optimal pH in the range of 6–8. The optimal buffering range for a buffer is the dissociation constant for the weak acid component of the buffer (pKa) plus or minus pH unit.

Is higher or lower buffer size better? ›

So, this is a balancing act: the smallest-number buffer size will be better, but it may tax your computer's processing power, resulting in errors. If you have a less powerful computer, you'll likely need to increase your buffer size, both while recording and mixing, to keep from encountering errors.

At what pH is a buffer most effective? ›

Buffers are generally good over the range pH = pKa ± 1. The ammonia buffer would be effective between pH = 8.24 - 10.24. The acetate buffer would be effective of the pH range from about 3.74 to 5.74. Outside of these ranges, the solution can no longer resist changes in pH by added strong acids or bases.

Why is bamh1 important? ›

Because of its ability to recognize specific DNA sequence and cleave by a nuclease, BamHI carries various importances in understanding Type II restriction endonuclease, cloning DNA, and possibly treating certain DNA mutation-derived diseases through genetic therapy.

Does BamHI produce sticky or blunt ends? ›

EcoRI and BamHI cuts their respective restriction sites to form sticky ends while HaeIII and AluI cut DNA strands to form blunt ends.

Is BamHI sticky or blunt? ›

Recognition Sequences
EnzymeOrganismBlunt or Sticky End
EcoRIEscherichia ColiSticky
BamHIBacillus amyloliquefaciensSticky
BglIIBacillus globigiiSticky
PvuIProteus vulgarisSticky
8 more rows

Can I leave restriction digest overnight? ›

Time-Saver qualified enzymes can cut substrate DNA in 5-15 minutes and safely digest overnight. For enzymes that are not Time-Saver Qualified, the recommended incubation time is 1 hr. In general, long incubations (several hours to overnight) are not recommended, unless digesting some gDNAs.

How long does it take to inactivate restriction enzymes? ›

Incubation at 65°C for 20 minutes inactivates the majority of restriction endonucleases that have an optimal incubation temperature of 37°C. Enzymes that cannot be inactivated at 65°C can often be inactivated by incubation at 80°C for 20 minutes.

How long does restriction enzyme last? ›

All enzymes are assayed for activity every 3-6 months; the most recent assay date is given on the label attached to each vial of enzyme. After thirty years of experience with restriction enzymes, we have found that most are very stable when stored at -20°C in the recommended storage buffer.

How do you run a restriction digest? ›

Protocol for DNA Digestion with a Single Restriction Enzyme

Incubate the reaction at digestion temperature (usually 37 °C) for 1 hour. Stop the digestion by heat inactivation (65 °C for 15 minutes) or addition of 10 mM final concentration EDTA. The digested DNA is ready for use in research applications.

Can you digest in 2 hours? ›

The normal range for transit time includes the following: gastric emptying (2 to 5 hours), small bowel transit (2 to 6 hours), colonic transit (10 to 59 hours), and whole gut transit (10 to 73 hours). Your digestion rate is also based on what you've eaten. Meat and fish can take as long as 2 days to fully digest.

How much DNA do you need for restriction digest? ›

Setting up a Restriction Enzyme Digestion

An analytical-scale restriction enzyme digestion is usually performed in a volume of 20μl with 0.2–1.5μg of substrate DNA and a two- to tenfold excess of enzyme. If an unusually large volume of DNA or enzyme is used, aberrant results may occur.

Is it OK to take 2 digestive enzymes? ›

Can more than one enzyme product be taken at the same time? Yes, but just make sure that you take digestive enzymes together with meals and therapeutic enzymes together on an empty stomach (30 minutes before or 2 hours after eating).

Can you use two restriction enzymes at once? ›

Digesting a DNA substrate with two restriction enzymes simultaneously (double digestion) is a common timesaving procedure. Over 210 restriction enzymes are 100% active in rCutSmart™ Buffer, making double digestion simple.

What are thermo scientific FastDigest restriction enzymes? ›

Thermo Scientific FastDigest Restriction Enzymes are an advanced line of enzymes offering fast and complete digestion of DNA in a single universal buffer.

What is the composition of tango buffer fermentas? ›

33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM magnesium acetate, 66 mM potassium acetate, 0.1 mg/ml BSA.

What are thermo scientific restriction enzymes? ›

Thermo Scientific FastDigest enzymes are a line of restriction enzymes that are all 100% active in a single buffer. The universal FastDigest and FastDigest Green Buffers allow single, double, or multiple DNA digestion within 5–15 minutes, eliminating any need for buffer changes or subsequent DNA clean-up steps.

How do Restrcition enzymes work? ›

How do restriction enzymes work? Like all enzymes, a restriction enzyme works by shape-to-shape matching. When it comes into contact with a DNA sequence with a shape that matches a part of the enzyme, called the recognition site, it wraps around the DNA and causes a break in both strands of the DNA molecule.

What are the 4 types of restriction enzymes? ›

Traditionally, four types of restriction enzymes are recognized, designated I, II, III, and IV, which differ primarily in structure, cleavage site, specificity, and cofactors.

What restriction enzymes are used in CRISPR? ›

CRISPR/Cas9 genome editing strategies often rely on the placement of an introduced restriction endonuclease (RE) site adjacent to the genomic edit of interest. This allows for rapid initial PCR-based detection of cells and organisms containing the edit of interest and may also be used for subsequent genotyping.

What are the three types of restriction enzymes? ›

Types of Restriction Enzymes
  • Type I. These restriction enzymes cut the DNA far from the recognition sequences. ...
  • Type II. These enzymes cut at specific positions closer to or within the restriction sites. ...
  • Type III. These are multi-functional proteins with two subunits- Res and Mod. ...
  • In Gene Cloning.


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